Reporter

Part:BBa_K270004:Design

Designed by: Ann Lesnefsky   Group: iGEM09_Michigan   (2009-10-19)


Pr XylR Pu GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1070
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 893
    Illegal NheI site found at 2622
    Illegal PstI site found at 1070
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 513
    Illegal BglII site found at 1975
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1070
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1070
    Illegal NgoMIV site found at 320
    Illegal NgoMIV site found at 679
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1166
    Illegal BsaI.rc site found at 3340


Design Notes

The Pr XylR must come first because it has an illegal PstI cutsite


Source

See Bba_K270001 and Bba_K270003

References